Respuesta :
I think the effect would be that the lactose transport will decrease. When glucose levels are high inside the bacteria, it leads to inhibition of adenylyl cyclase and lactose permease, therefore cAMP levels are low and lactose can not be transported within the bacteria. Once the glucose is used up, the second preferred source of carbon is lactose, thus absence of glucose will switch off the catabolite repressor.
There will be a decrease in lactose transport due to the presence of high glucose levels.
Further explanation:
The lac operon has genes for lactose metabolism in bacteria. The prime condition for the proper functioning of the lac operon is the high levels of lactose and absence or less amount of glucose in the bacterial cell.
There are three genes present in the lac operon to metabolize lactose. It also contains catabolite activator protein (CAP) and lac repressor which are responsible for sensing levels of glucose and lactose, respectively. The lac repressor usually inhibits the operon but acts opposite in the presence of lactose. The CAP protein activates the operon only in the absence of glucose.
The E. coli or bacteria utilize glucose and lactose as energy source. The breakdown of glucose involves fewer steps and comparatively less energy, therefore glucose is the prime source of energy. In glucose absence, the bacteria use lactose for energy.
When lactose is abundant, the enzyme galactosidase permease helps in the transport of lactose inside the cell and the operon functions for the transcription of lactose. A low glucose level also activates lac operon as during this time high levels of cAMP are present which binds to CAP and the dimer helps in the proper binding of RNA polymerase to the lac promoter. This, in turn, activates the lac operon.
In the presence of glucose, cAMP cannot be activated due to the inhibition of adenylyl cyclase and also galactosidase permease is inhibited which resists the transport of lactose inside the cell. As a result, lactose transport is reduced and also glucose is utilized because of its abundance.
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Answer details:
Grade: High School
Subject: Biology
Chapter: Lac Operon
Keywords:
Lac operon, lactose, galactosidase permease, glucose, cAMP, adenylyl cyclase, transcription, CAP, lac repressor.
