Step 1: A lily leaf is taken and simply fold in the centre which gets broken into two pieces.
Step 2: One leaf piece is gently pulled and we got a transparent leaf peel.
Step 3: The leaf peel of lower epidermis is taken and cut with scissors or a blade into small pieces. These pieces transferred with the help of paintbrush into a petri dish containing water. (Do not keep them without water. They will dry)
Step 4: A piece of leaf peel is selected and transferred into another watch glass containing dilute solution of safranin (a red stain).
Step 5: With the help of paintbrush specimen is transferred back into first water containing petridish/watch glass to remove excess of stain.
Step 6: Specimen is transferred to slide with the help of brush.
Step 7: A drop of glycerine is put on the peel and it is covered carefully with a cover slip by using needle to avoid air bubbles. Excess glycerine is removed with help of a filter paper.
Step 8: The leaf peel is observed under low power of microscope.
Step 9: For inner details, again the leaf peel is observed under high power (45x) of microscope.
Hope this helps!! ;))
Have a grat day!! <3
