In substrate-level phosphorylation, a molecule donates a phosphate group to ADP to form ATP, and a proton gradient is not required.
What is Substrate-level phosphorylation?
- Substrate-level phosphorylation is a metabolic reaction that results in the production of ATP or GTP by the direct transfer of a phosphate group from a substrate to ADP or GDP.
- This process uses some of the released chemical energy, the Gibbs free energy, to transfer a phosphoryl (PO3) group to ADP or GDP from another phosphorylated compound.
- This occurs during glycolysis and the citric acid cycle.
Therefore, in substrate-level phosphorylation, a molecule donates a phosphate group to ADP to form ATP, and a proton gradient is not required.
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