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It might be explain by factor that In blood, the concentration of H+(aq) is maintained at low levels by other equilibria.
One HA unit within the haemagglutinin titration is the minimum amount of virus that will cause complete agglutination of the red blood cells. The last well that shows complete agglutination is that the well that contains one HA unit. The HA titre is that the reciprocal of the dilution that produces one HA unit.
Why we perform HA test?
Hem-agglutination may be a widely used assay for detecting and titrating the influenza virus. One major advantage of this assay is that no specialized equipment is needed to perform the test. Although comes with relatively lower sensitivity, the assay are often used for robust and rapid detection of viruses.
What does hemagglutinin mean?
hemagglutinin, any of a gaggle of naturally occurring glycoproteins that cause red blood cells (erythrocytes) to agglutinate, or clump together. These substances are found in plants, invertebrates, and certain microorganisms.
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