A protein-containing solution changes color when biuret reagent would be added, turning pink or purple. The solution glows blue when there is no protein present.
The test substance is first liquefied as well as dissolved if it becomes solid, such as food. The tester next carefully adds just few drops of a 1 percent copper sulfate aqueous solution without shaking the mixture. Protein inside the sample is indicated by the color violet.
Lack of violet color development as a result of the test could indicate a protein deficiency or the existence of free amino acids. However, any molecule with two carbonyl groups joined to nitrogen as well as a carbon atom yields a positive response for the test.
Therefore, A protein-containing solution changes color when biuret reagent would be added, turning pink or purple. The solution glows blue when there is no protein present.
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